These comparisons provide the opportunity to assess the ability to leverage the genomic information from highly characterized plant genomes for application in other less well-characterized species.
For example, since highly characterized autoantigens are a key to high performance immunoassays, an apparent oversight in this study was the lack of a detailed description of the recombinant PM/Scl-100 antigens that were produced in bacteria or insect cells (for example, purity or presence of a fusion-tag).
Subsequently, in order to determine the genome level differences between the model carrier strain and non carrier strain, we undertook a complete genome sequencing effort for our two highly characterized strains, D30 (carrier) and 930918-3 (non-carrier), and identified differential gene complements in both strains.
Each donor-recipient (D-R) pair of genomes contains vast permutations of non-synonymous amino-acid differences and other potential sources for allogenicity, beyond the highly characterized human leukocyte antigen (HLA) region, conventionally considered to contain the main genetic factors underpinning allograft rejection.
Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A highly characterized yeast toolkit for modular, multipart assembly.
For comparison with a positive control, we also cloned a SV40 NLS, a highly characterized NLS motif, similar to Tus NLS with GFP.
In summary, these highly characterized PDX models serve as a renewable resource of human breast tumor tissue and preclinical models for preclinical evaluation of novel cancer therapies.
As VEGF antagonism has become the mainstay of treatment for highly prevalent retinal diseases characterized by neovascularization and retinal vascular leakage such as neovascular AMD, diabetic macular edema, and retinal vein occlusions, recent attention has focused on the associated risk of infectious endophthalmitis with intravitreal injection of these agents.
A novel uncertainty propagation method for problems characterized by highly non-linear or discontinuous system responses is presented.
We [ 21] and others [ 22] previously characterized genes for highly related proteins in X. laevis, M. musculus and H. sapiens (named nocturnin, "mCCR4" and "hCCR4"), disclosing circadian expression [ 22], and with C-terminus displaying significant similarity with the C-terminus of yCCR4 (close to 30%).
