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Electron microscopy allows for higher magnifications.
For higher magnifications the mineralogist uses an electron microscope, which produces images with diameters enlarged tens of thousands of times.
For higher magnifications, an Axioskop 40 FL upright microscope (Carl Zeiss, Oberkochen, Germany) equipped with Achroplan, Fluar and Plan-Neofluar objectives of up to 100× magnification was used.
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Optical zoom was used for higher magnification image.
High-resolution image acquisition was performed by slow unidirectional sequential scanning (200 400 Hz; beam expander: 6) and smaller frame sizes were used for higher magnification.
For higher magnification, samples were observed with an Olympus BX60 microscope (Olympus, Tokyo, Japan) interfaced with a ProRes© Capture Pro 2.1 camera and software (Jenoptik, Jena, Germany).
Pollen tube length and tip morphology were examined at various time points (2 to 16 h) using a Leica dissecting microscope for higher magnification.
Slices were mounted with Vectashield mounting medium (Vector Laboratories, Burlingame, CA) and detection of EYFP or tdTomato expression was performed using a Leica M205-FA for low-magnification images, and a Leica TCS SPE confocal microscope under a 20X objective for higher magnification images.
The need to use additional elements in the lens system for high magnifications further shortens the working distance to only 10 to 20 percent of the focal length.
Use of other distances will affect the aberration balance for high-magnification objectives.
Immunochemically functional quantum dots have been used for high magnification, three-dimensional erythrocyte reconstruction [57].
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