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Urotropine was used as an etalon for higher confidence.
This allows for higher confidence in target discovery and pathway studies.
The observed error frequency declined more slowly for higher confidence scores.
For higher confidence, only the clean reads with read count ≥ 2 were included in further analysis.
We employed a normalization method to select for higher confidence 3'UTR isoform switching events based on the ratio between PAS coverage (see Methods).
However, the threshold is flexible and can be increased for higher confidence in Y-linkage or decreased for a lower rate of false negatives.
This allowed for higher confidence in breakpoint assignment and in deciding whether a CNV in Qataris was observed previously or not.
For higher confidence, only the unique reads with read count equal to or larger than two were used for mapping back to human pre-miRNAs (miRBase 16).
Moreover, for higher confidence, only the unique clean reads with read count ≥ - 2 were mapped back to human pre-miRNAs (miRBase 16).
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The reported work is prospective and is meant to show the feasibility of such a technique for high confidence trustful devices.
For masses in which MDR activity outweighs the presence of mitochondria, the tumors appear cold on 99mTc-sestamibi SPECT/CT, allowing for high confidence in the diagnosis of renal cell carcinoma.
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