Sentence examples for for high transformation from inspiring English sources

For planning and policy this means not only considering transformation rates as such but also looking more closely at spatial and contextual specifics in order to be able to account for the susceptibility of spaces or elements for high transformation rates.

The TtPilF (PilF ATPase from Thermus thermophilus) is required for high transformation efficiency, but its mechanism of action is unknown.

Therefore, isolating DNA from a dam+ dcm- E. coli strain such as BL21 allows for higher transformation efficiency overall while also eliminating the plasmid-to-plasmid variation observed when DNA was isolated from E. coli Top10.

In addition, the length of co-culture during the transformation was also important for obtaining high transformation efficiency, as the bacteria were observed to be completely removed from the suspension cells after 3 d of co-culture (data not shown).

The reason for a high transformation efficiency may be attributed to optimized regeneration protocol and the effective selection procedure employed for the recovery of transformants (8 10 weeks following particle bombardment).

Whether PilQ in Thermus is responsible for particularly efficient DNA uptake and high transformation rates is questionable.

By varying the number of the control and check points it is shown that increasing the number of the control points more than three well distributed is not relevant for significantly higher transformation accuracy.

Here, we outline the procedures for high-efficiency transformation using liposomes, gene expression from a plasmid, and exploitation of homologous and site-specific recombination to add and delete genes from the chromosome.

This study aimed to determine whether SOX2 (sex determining region-Y-related high mobility group box 2), a protein expressed in the epithelial basal proliferative zone in dentigerous cysts, is a marker for ameloblastic carcinoma as well as for high-grade transformation in ameloblastic neoplasms.

This strategy involved ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via vector-backbone exchange (VBEx) into an organism-specific plasmid ready for high-efficiency transformation.

Overall, optimum values of 30 kDa and 1 may be suggested for the molecular weight and N/P to obtain size of 125 nm for pDNA/chitosan nanoparticles, with highest transformation efficiency (Fig. 3).