We successfully found an appropriate centrifugal acceleration condition to obtain GUVs that had a desired size and narrow-enough size distribution with an improved yield so that emulsion droplets can become the template for GUVs.
Arbitrarily selected micrographs from a time-lapse sequence of fluorescence images from Video 4 for GUVs prepared by 'gentle hydration'.
Although the absolute bending rigidity is not comparable, it is evident that bending rigidity measurements can be obtained for GUVs made from whole-body extracts with at least the same precision as for POPC GUVs.
For GUVs of ternary lipid mixtures of unsaturated DOPC, saturated SM and cholesterol, the spectral GP image analysis reflected the expected phase separation into Ld and Lo domains on a single GUV, with the GP values of the Lo and Ld environments depending on the ratio of the lipid mixture.
Soc Gen claimed whatever the French for "Us, guv?
Note that no lysis or membrane dislocation of GUV was observed for any condition, even at the highest ratio (data not shown).
Here, we present a method for fabrication of GUVs with different, osmotically-balanced solutions on the interior and exterior of the bilayer.
A DeltaVision microscope (Applied Precision, Inc., Washington, USA), fitted with a PLAPON 60XO/1.42 NA oil-immersion objective from Olympus and DAPI, TRITC, FITC, and CY5 Semrock filters (New York, USA), was used for imaging of GUVs in real-time using wide-field deconvolution fluorescence microscopy.
GUVs were then formed by the dehydration-rehydration method, which previously has been used for creating GUVs containing functional membranes with functional ion channels, used for patch-clamping [20].
For typical GUVs, the intensity of red fluorescence emitted from the membranes is about 25 units.
