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Further indications for good normalization are the distributions of between (MSQbetween) and within (MSQwithin) group variances and their relation to each other.
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For a good normalization scheme, the estimates of the location and scale parameters of the matching score distribution must be robust and efficient.
In practice, the AC score achieves a good normalization for the pre-scores of different miRNAs.
Good normalization procedures for miRNA expression are still lacking.
A second reason for comparing the miRNA expression profiles by using alternative profiling techniques is related to the fact that good normalization procedures for miRNA expression data are currently still lacking.
A good normalization method should result in similar expression values for replicates and thus in small MSQwithin values hardly crossing this artificial MSQbetween.
However, good normalization protocols still have to be implemented to facilitate data exchange and comparison.
Therefore, cdc2 expression levels provided a good normalization baseline.
Therefore, specific markers and good normalization procedures are needed.
These results show a good efficiency for the normalization step and demonstrate the convenience of this approach to improve the efficacy of the sequencing effort at isolating new and/or low expressed transcripts in the EST collections.
From microarray experiments, genes, which display stable expression across all fold-changes are likely to be good candidates for normalization for qPCR.
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CEO of Professional Science Editing for Scientists @ prosciediting.com