A new regulatory framework for gain-of-function research could set a significant precedent by creating a new standard for oversight in the life sciences.
Because of the ease of using lesion length as a quantitative trait, our initial screen for gain-of-resistance mutants was based on reaction to bacterial blight.
The Nav1.8 sequence has recently been studied for gain-of-function mutations which have been linked to painful peripheral neuropathy [50].
On the other hand, certain effects of FHM2 mutations argue for gain-of-function activity [51].
As many current anthelmintics, including PZQ, are agonists, it would be worthwhile developing systems for gain-of-function mutants in model organisms (for example using targeted overexpression banks).
As an alternative we engineered the Lyme disease spirochete B. burgdorferi to express Vsp1 or Vsp2 in the outer membrane for gain-of-function experiments.
We have reviewed evidence for gain-of-function and loss-of-function mechanisms in C9FTD/ALS.
Support for gain-of-function mutations in GBA is mainly from the following findings.
In contrast, RGOH-M for gain-of-heterozygosity was merely 1.54 × 10−3 % of all MM residues analyzed.
