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The ESTs reported will provide an excellent resource for future assembly and annotation of transcriptome sequences from the NGS platforms, as well as for annotation of the spruce whole genome sequences.
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In addition, it constitutes the first direct comparison between two of the most widely used assembly programs in a polyploid organism, and thus provides guidelines for future assemblies.
This map provides an improved tool for genetic mapping and comparative genomics in mink, also useful for the future assembly of the mink genome sequence when this will be taken forward.
These factors should be taken into consideration for any future assembly attempts on polyploid species and the methodology applied for the assembly of an allopolyploid transcriptome in this study could be useful for guiding future genome assembly work in polyploidy.
High density linkage maps are presently being constructed, using genotyping-by-sequencing methods (Elshire et al. 2011); these maps should provide critical information for improving future assemblies of the Pacific oyster genome, as they have in other species (e.g., Dalloul et al. 2010; Dodgson et al. 2010).
The majority of genes in the current draft genome were placed on a LG and at their approximate chromosomal position, resulting in the first chromosome-scale assembly of cassava and a map that will serve as a valuable guide for future genome assembly improvements.
As high-throughput sequencing technology continues to reduce the costs of generation of DNA sequences, we expect the availability of the large transcriptomic dataset presented here to be useful for future genome assembly and gene prediction efforts.
Consequently, this study highlights regions for future genome assembly improvements.
The ER:mitochondrial contacts mark the site for future Drp1 assembly and subsequent completion of the division process.
Future assembly plants need to utilize flexibility systematically, both for the system design and its operation.
As researchers continue to work towards elucidation of the crocodilian genome, this first generation map lays the groundwork for more detailed mapping investigations, as well as providing a valuable scaffold for future genome sequence assembly.
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