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These annotation errors need to be minimized because high-throughput gene calls are submitted to public databases, which in turn provide evidence for future annotations.
Unfortunately, these dubious genes polluted public repositories, acting as a source for future annotations.
The normalized syntax will allow exchange of data with other databases, reduce redundancies, and provides a framework for future annotations.
The transcriptome will be important for gene discovery in Cucurbita and for future annotations of the Cucurbita genome sequence.
Therefore, this resource will enhance comparative studies within the family and the transcriptome will serve as a starting point for gene discovery in Physalis and for future annotations of the Physalis peruviana genome sequence.
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We discuss the impact of our findings on strategies for future annotation efforts and parser evaluations.
Based on this experience, we suggest some guidelines for future annotation of metabolites in metabolic network reconstructions.
Note that the number of lines to skip might differ for future annotation files.
Transcriptomic datasets like this will be a useful contribution for future annotation of the F. vesiculosus genome (http://www.cemeb.science.gu.se/research/imago-marine-genome-projects/).
This study is among the first reported marsupial EST datasets with a significant inter-species genome comparison between marsupials, providing a valuable resource for transcriptional analyses in marsupials and for future annotation of marsupial whole genome sequences.
The results are evaluated for improving the instruction on the webpage and annotation tool for future annotators.
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