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Half of the lungs were removed, homogenized in DMEM with 10% FBS, then frozen for future DNA extraction and quantitative real time PCR.
The buffy coat layer is stored for future DNA extraction.
Saliva specimens from infants and parents are collected at study entry for future DNA extraction.
The EDTA-containing tube is inverted several times and stored at -80°C for future DNA extraction.
If consent was given for genetic studies, a buffy coat sample was banked for future DNA extraction.
However, brain tissue homogenate of positive mice were kept deep frozen for future DNA extraction and genotyping elsewhere.
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Several worker and soldier termites and some comb material were preserved in 96% alcohol for each nest, and stored at 4°C for future reference or DNA extraction.
Cells were trypsinized at 24 hourly time points, over a total time-course of 72 hours, washed in PBS, and pellets were frozen at −80°C for future RNA and DNA extraction.
This technique is highly sensitive to low levels of 0.01 ng/mL/hour and is precise to activity that is less than 10%[ 91] In addition, a blood sample is drawn for storage and DNA extraction for future genetic studies.
Written informed consent was obtained for participation in each trial and for the collection of blood for DNA extraction for the future studies of genetic variants.
In addition, patients are requested to provide a sample of blood for DNA extraction and genotyping for future pharmacogenetics research.
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