Sentence examples for for further washing from inspiring English sources

The supernatant was carefully decanted, and the residual precipitate was dispersed in ethanol and prepared for further washing.

New PBS was added to the biopsies for further washing.

For further washing from salts, 0.5 mL Amicon Ultra centrifugal filter units (Ultracel 30K, Merck Millipore Ltd).

A lipid buffer with monovalent cations (10 mM HEPES, 150 mM NaCl, pH 7.6) was used to dilute cholesterol-modified oligonucleotides and for further washing steps.

PBS/T was then used for all further washing steps.

Cells on day 10 were stimulated with peptides, or were unstimulated, for 20 hours, the last 18 hours of which were in the presence of 0.1 µg/ml Brefeldin A. Cells were washed, stained with surface antibodies (CD3, CD4, CD8) for 20 minutes before further washing and addition of Perm/Fix for 20 mins.

Cells were incubated at 4°C for 20 minutes before further washing in FACS buffer.

Species-specific fluorochrome-conjugated secondary antibodies (Invitrogen) were then applied for 1 2 hrs, before further washing and mounting in Vectashield mounting medium (Vector Laboratories , Inc. containing 100 ng/ml 4,6-diamidino-2-phenylindole (DAPI).

The reaction was continued for 2 h before further washing.

Following further washing for 24 h in 10% buffered formal saline, tissue samples were processed and embedded in paraffin wax.

After a further washing for 3 × 5 min in TBS with 0.1% IGEPAL CA-630, the cells were mounted on slides with VECTASHIELD (Vector Laboratories, Burlingame, CA, USA).