Sentence examples for for further two hours from inspiring English sources

Subsequently, 200 µl of pronase (500 µg/ml), DNase (90 units), or sodiummetaperiodate (10 mM) diluted in PBS were added into the wells and the microtiter plates were incubated for further two hours at 37°C under anaerobic conditions.

Then isopropyl thio-β-d-galctoside (IPTG) was added to a final concentration of 1 mM and cultures were incubated for further four hours.

Lower the heat to 170C/325F/gas mark 3, and cook for a further two hours, basting every 20 minutes.

Expression of proteins was induced with 0.1 mM IPTG at 30°C for a further two hours with shaking.

We noticed that there were higher frequencies of cells with separated sisters in the zero time point samples than in the samples that had been incubated with nocodazole for a further two hours (Fig. 1D).

The reaction mixture immediately turns red-brown and was stirred for a further two hours.

Ten microlitres of WST-1 were added to each well, and the cells were incubated for a further two hours.

An equal number of myoepithelial cells labelled with red cell tracker was then added to the luminal population and cells aggregated for a further two hours.

Following digestion, 2 μL of Calf Intestinal Alkaline Phosphatase (Promega, Madison, WI) was added and incubated at 37°C for a further two hours.

Cells were grown at 37°C until the OD600 reached 0.4 0.6, and protein expression was induced by adding IPTG (0.5 mM) and incubating for a further two hours.

Five patients with active disease were treated with Infliximab, 5 mg/Kg, by a two hour infusion, at weeks 0, 2, 4 and the patients observed for a further two hours without adverse effects.