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We used dental cement for further fixation, and sutured the incision.
Angle-stable 3.5-mm screws were used for further fixation (Fig. 3).
For further fixation the brain hemispheres were transferred to 20%% sucrose solution and fixed overnight at 4 °C.
In one case, aseptic loosening of the symphyseal plate was detected at 2-month follow-up; the hardware was removed 10 days later, without need for further fixation.
The vitelline membrane was then carefully removed and the embryo together with attached yolk was transferred to 4% PFA for further fixation and storage at 4°C.
The femoral shaft of the proximal part was placed in special containers filled with Technovit 3040 (Heraeus, Wehrheim, Germany) for further fixation.
Similar(53)
The cornea was cut and the lens was removed, to allow further fixation for 1 hour.
Samples were fixed in 4%PFA/0.1%% gluteraldehyde in PBS for 30 minutes followed by further fixation in 4% PFA for one hour, rinsed in PBS, scraped and re-suspended in 10% gelatin.
Biopsies will be gently extracted and sent to the site's laboratory for further processing (fixation and paraffin wax embedding).
Tissue was dispersed with a hand-held homogenizer and further fixation of retinal tissue was continued for an additional 30 min on ice.
For the detection of S phase cells, a 1 h BrdU (bromodeoxyuridine) pulse was followed by fixation, immuno-detection of green fluorescent protein (GFP), further fixation, acid treatment and immuno-detection of the BrdU epitope.
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