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The sample was then centrifuged at 4000, r.p.m. for 10 min: the cellular debris pellet was kept for further extraction, while the supernatant was extracted with 2.5 ml chloroform/isoamylalcohol (24/1) and the DNA in the upper phase was precipitated by addition of 2 volumes Solution II (1% w/v CTAB, 50 mM Tris HCl (pH 7.6), and 10 mM EDTA) and centrifugation at 8500, r.p.m. for 10 min.
The processed drugs were powdered to uniform particle size of (mesh size 2 8) for further extraction and analysis.
Three different standards (0.05, 0.10 and 0.20 mg mL−1) were added to blank sample separately for further extraction and analysis.
Similarly, at the receiver side, the process of rebuilding these frames is done in the virtual memory space and then passed to the decoder for further extraction.
Over the years, prime-coking coal reserves in the Jharia Coal Field have been exploited from the upper seams, leaving behind lower seams of inferior grade coal for further extraction.
It can be seen from the comparison of the two figures that the algorithm proposed in this paper can restore the micro-Doppler signal caused by the heartbeat and breathing effectively, which may lay the foundation for further extraction of the signal parameters.
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A chad was punched from the sampling reservoir and inserted into a LOC well containing the separation buffer for direct analysis, avoiding any further extraction step.
For the Triton-insoluble fraction, further extraction was performed with 1 M NaCl, followed by centrifugation at 150,000× g for 35 min at 4C.
Since somatic cells remain in the Matrix-Lysis pellet and result in high background DNA levels and overload of the DNA extraction column, only 1/2 of the B3 buffer lysate was used for further DNA extraction.
Furthermore, creating a supply of living plants for trade is critical to avoid further extraction of plants from nature.
All articles from January 1970 onward that presented data concerning treatment of nonunions of femoral shaft fractures were included for further data extraction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com