Exact(31)
The over expression of P-selectin facilitates further attachment of EV allowing for further expression of its tissue factor.
An engineered TEV protease lacking the C-terminal residues 238 242 was then used for further expression optimization.
The pGEX-6P1-hCypB expression vector was constructed by PCR amplification of hCypB cDNA using specific primers (5'GGATCCGCCGATGAGAAGAAGAAGGG-3' and 5'CCCGGGAAAGATGTCCCTGTGCCCTA-3') and ligated into the BamHI-SmaI restriction sites of pGEX-6P1 (Amersham) for further expression and purification of recombinant GST-hCypB fusion protein.
We included only those 31 miRNAs for further expression analysis.
Based on these results, RMA processed data were used for further expression analysis described below.
As these genes are closely related, only one of each was followed for further expression characterization.
Similar(29)
Further, expression profile for piRNA in different tissues and from different developmental stages has been provided.
The data are consistent with our digital gene expression database, and Actin1 was chosen as the reference gene for further gene expression analyses.
The cloned genes were inserted into the prokaryotic vector pET28a or the eukaryotic vectors pEGFP-C3, pEGFP-N1 and pcDNA-3.1 N-Flag for further exogenous expression in E. coli and human cells.
Only pairs of oligonucleotides with efficiencies between 90 110% were accepted and the efficiency was considered 100% for further relative expression quantifications.
Apart from two transgenic T1 lines exhibited poor fertility, 24 independent T2 transgenic A. thaliana lines were performed for further gene expression analysis with RT-PCR.
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