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Only high-quality reads (q-value > 25) passed the filter for further assembly and they were assembled using Newbler version 2.5.3 program (454 Life Science-Roche) with the parameters by default with the cDNA option.
These were brought to a yard on site for further assembly into modules that were then finally constructed into the finished structure.
Reads that did not fully map to over represented regions were combined with the contigs covering these regions in order to produce a bioinformatically normalized dataset for further assembly.
For further assembly details see Additional file 3: Table S2.
Finally the reads with at least 90% bases > quality 20 were chosen using fastq quality filter for further assembly.
In these examples, structured domains mediate specific interactions to form assemblies that serve as scaffolds for further assembly of components of membrane-less organelles.
Similar(41)
The kmer length of 41 was selected for further assemblies.
Besides providing information on the shape of individual, isolated amelogenin nanoparticles, SAXS results have also allowed us to identify the conditions for the further assembly of the nano-oblates into high-order elongated structures.
Above a certain threshold of assembly, AChE586-599 aggregates would eventually relocate to the bulk solution to free the air-water interface for monomer recruitment and further assembly.
According to the method of Brassica rapa physical map assembly, the fraction of clones assembled (71.4%) was sufficient to give a robust basis for the further assemblies [ 36].
Thus in the second round of selection, S t r i c t- T parameter was used for two further assemblies that had relaxed minimum molecule length (R e l a x e d- M i n l e n) of 100 kb rather than the 150 kb default or a strict minimum molecule length (S t r i c t- M i n l e n) of 180 kb.
More suggestions(15)
for successive assembly
for subsequent assembly
for further recalls
for further commitments
for further combined
for future assembly
for further articles
for further components
for further matters
for further remarks
for further flattening
for peaceful assembly
for further study
for further review
for different assembly
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