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After competitive binding in a sample solution containing respective monoclonal antibodies, secondary antibody-conjugated AuNPs are employed to bind with the captured monoclonal antibodies for further amplification of the SPRi signal.
Additional specific primers were designed based from sequenced regions for further amplification and confirmation of HP, SERA genes and hesB (Figure S5).
Once formed, the hairpin structure hinders the primer annealing for further amplification and therefore primer dimer, if formed, could only be produced in a linear way [10].
The rest of the sample was stored at -80°C for further amplification and sequencing.
The amount of dNTPs carried over from the reverse transcription reaction is fully sufficient for further amplification.
Positive clones were plaque-purified twice and a single plaque was chosen for further amplification in 293 cells.
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For the sample YAKa28 it was not possible to remove the inhibitors and therefore no results were obtained for further amplifications.
All PCR products were purified using the QIAquick PCR Purification Kit (Qiagen) and diluted (final amount: 500 pg) for further amplifications.
Dept of Further Amplification.
By William J. Fielding The New Yorker, August 2, 1952 P. 44 Dept of Further Amplification.
He also gives a kicking to newspapers and conventional broadcasters who, scared of looking backward and hungry for free content, give further amplification to the howlround.
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