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Synthetic promoters and introns provide useful approaches for functional validation of promoter sequences.
As such, differential expression of core TF genes does not necessarily link SCs with cancer, thus underscoring the need for functional validation of all potential biomarkers.
The pCAMBIA2301-35S SbSOS1 construct was introduced into tobacco plants for functional validation of SbSOS1 gene.
Such changes provide targets for functional validation of molecular mechanisms of disease progression.
However, they represent an entry point for functional validation of candidate molecular genetic mechanisms underlying established QTLs.
Our comprehensive analyses will help design experiments for functional validation of their precise role in plant development and stress responses.
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These results can be used for functional validation studies of the rice OSCA genes and increase our understanding of the roles of plant OSCAs.
The findings in our work would be useful in selecting candidate genes for functional validation studies of PTR members in rice.
These investigations add to our understanding of the importance of OsPTR family members and provide useful reference for selecting candidate genes for functional validation studies of this family in rice.
The questions that surround the strategy for functional validation are part of an ongoing dialog between two apparently diametrically opposed experimental philosophies in modern microbiology: the 'top down' unbiased, genomics-based approaches (which include GWASs and other experimental strategies [ 59– 62]), and the 'bottom-up', gene-by-gene approach of classical molecular genetics [ 6].
It will also allow for the development of appropriate transgene constructs for the functional validation of candidate genes or sequences.
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