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Using the pollen tube as a model for functional tests, we verified the function of three candidate genes and illustrated their role in pollen-tube tip growth (Additional file 13: Table S12).
In the above model 33, lung neutrophils are elicited for functional tests using LPS aerosolization, which may affect their function.
Conical couplers were designed and fabricated to fit a 3.5-MHz, spherically concave transducer for functional tests, including Schlieren imaging, power efficiency measurements and in vivo hemostasis experiments.
For functional tests fly heads were collected as previously described [16] and membranes were prepared for radioactive glutamate binding assays.
As positive controls for functional tests rat primary hepatocytes were isolated from 5 weeks old Fischer rat using a two-step collagenase-perfusion method [50].
For functional tests of the A33 binding IgG-LC-LPETGGS fused to GFP, 1 µl of Dynabeads® Biotin Binder magnetic beads (Dynal, Invitrogen, Carlsbad, CA) was washed twice in phosphate buffered saline, 0.1% bovine serum albumin (PBSA), and incubated with 250 µl of 850 nM biotinylated A33 antigen (biotinylation reagent, Pierce, Rockford, IL) in PBSA at 4°C overnight on a rotator.
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The requirements for functional testing of devices and distributed functions also determine the methods for testing of both types of systems are proposed based on the following order of system components tests: Functional testing of individual IEDs used in the scheme Functional testing of distributed functions within a substation.
b, A comparison of the 14 disparate RT Cas1 proteins selected for functional testing.
In order to ensure the system's compliance with requirements, adequate solutions for functional testing are needed.
After further studies to identify gene clusters that are evolutionarily conserved across multiple genomes and in a broad distribution of microbes, they selected 28 such clusters for functional testing.
Currently there are alone more than 28 tools and frameworks for functional test automation and more than 16 different device clouds available.
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