Our approach enables the extensive study of T cell function and also the ability to recapitulate TCRs for functional studies and therapeutic application.
Our work provides clues for functional studies that will aim at showing gain or loss of function.
Lyko, F. & Maleszka, R. Insects as innovative models for functional studies of DNA methylation.
It is especially suited for functional studies, yielding valuable results with minimal side effects.
Swamydas M, Lionakis MS. Isolation, purification and labeling of mice bone marrow neutrophils for functional studies and adoptive transfer experiments.
De Rybel, B. et al. A versatile set of ligation-independent cloning vectors for functional studies in plants.
However, optogenetic applications in vitro are severely limited, as obtaining the expression levels required for functional studies is not trivial.
Swamydas, M. & Lionakis, M.S. Isolation, purification and labeling of mouse bone marrow neutrophils for functional studies and adoptive transfer experiments.
Using such lines for functional studies requires a method that transforms GFP into a molecule that enables genetic manipulation.
They were specifically designed to enable construction of asymmetrically-mutated variants suitable for functional studies.
However, recent advances in fluorescent dyes and optical recording methods provide new opportunities for functional studies of the retina, with many advantages over purely electrophysiological methods.
