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Gene expression was also monitored in the presence of cisplatin, in preparation for functional experiments.
Conversely, the scarce availability of antibodies in teleost research to date often hampers the opportunity for functional experiments.
Demonstration of similar deleterious effects of the elongated mutant protein could pave the way for functional experiments intended to clarify some basic biochemical features of GP-9 protein.
One of these microRNAs, miR181a, was selected for functional experiments given its role in proliferation and suppression of the cell cycle inhibitor p27 in human myeloid leukemia cells [33].
Additionally, we also used the offspring of UASdmDysC-term crossed with the driver 24B-Gal4 (musclerm musclexpressionof of dsRNA) and UASdmDysN-term (expression of dsRNA to block the expression of all short and long dystrophin isoforms) driven by both drivers for functional experiments.
These patients are different from the ones used for functional experiments and represent a retrospective cohort.
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The onset of differentiation after 5 days of culture and the gradual loss of Sca-1, c-Kit and Musashi-1 expression after 3 days of culture indicated that for subsequent functional experiments employing transplantation, cells that had been cultured for 3 days were most suitable.
The MDA-MB-453 line was used for the functional experiments since it represents a widely accepted cell line model for molecular apocrine subtype [ 5, 10- 12, 33].
The positive γδ T cells were isolated by flow sorting for further functional experiments.
Line2 and Line2-T500 were transfected with siRNA using Lipofectamine RNAiMAX in the same ratios as for the functional experiments except in 6 well plates and after 36 hours, the RNA extracted using the GeneJET RNA purification kit (Fermentas) according to the manufacturer's instructions.
Moreover, the information provided on key genes may serve as basis for further functional experiments.
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