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From the part of the bladder designated for functional examination, full thickness transverse strips were prepared (6 × 2 mm).
For functional examination of the differentially expressed genes identified in this study, we looked for association of SNPs with photosynthetic traits.
However, the necessity for functional examination of cis-regulation in the appropriate tissue has also been highlighted by the observation that in approximately 50% of differing tissues the same haplotype has differing effects [ 18].
Furthermore, we selected six candidate genes for functional examination by a single-marker association approach, which demonstrated that 20 SNPs in five candidate genes significantly associated with photosynthetic traits, and the phenotypic variance explained by each SNP ranged from 2.3%to12.6%6%.
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From the sacrificed rats, the urinary bladder was excised and thereafter divided into two identical parts; one half was used for functional examinations, while the other half was used for morphological and biochemical examinations.
Advances in modern sequencing technologies, bioinformatics analysis tools, and environmental DNA cloning methods should allow for a movement in natural products discovery away from a random microbe screening approach to a more systematic sequence-based screening approach that will allow for the functional examination of gene clusters that remain hidden in the environment.
In order to include high-order neighbors for functional neighbor examination, one may consider the concept of shortest path in the graphic theory and measure the distance of two genes as the length of the shortest path between them.
As it has already been shown that the first functional examination of a subject is not necessarily reliable for a control group, the whole examination was repeated twice, taking into consideration only the values obtained in the second trial.
The functional examination was done by using three scales.
Both genetic and functional examination identified one domain in the N-terminus of HBV preS1 (amino acids 1 47) necessary for infection.
The detailed additional physical examination consisted of further questions about symptoms, palpation and functional examination of the actual anatomical region.
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