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This conclusion is at odds with some previous reports that also used pharmacological agents to assay for functional contribution of BK and/or IK1 to proliferation.
Often, the boundaries of a new domain are loosely defined through mutagenesis experiments, as it is too time consuming to examine every amino acid near the suspected boundary for functional contribution.
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We note that, simultaneous with the present study, a group led by Drs. Khalid Meksem and Melissa Mitchum has completed tests for functional contributions of the Rhg4-locus LRR-K gene to SCN resistance and, similar to our work, has not detected a contribution for this Rhg4 candidate gene (K. Meksem, personal communication).
Pathogenic mtDNA mutations may indicate for their functional contribution in progression of the tumors.
We therefore question whether frequencies of occurrences are reasonable evidence that the structures of motifs have been selected for their functional contribution to the operation of networks.
It is notable that more than ten years after the filing of patent applications claiming SCN resistance function for the Rhg1-locus LRR-K gene, no groups have published evidence (beyond its presence as one of many candidate genes at the Rhg1 locus) for a functional contribution to SCN resistance by the Rhg1-locus LRR-K gene.
Furthermore, various host factors interacting with CA have been implicated in the early phase of HIV-1 replication, again arguing for a functional contribution of CA at this stage (reviewed in Matreyek and Engelman (2013); Ambrose and Aiken (2014); Hilditch and Towers (2014)).
For example, the functional contribution of the CID is not clear in the case of S. marcescens chiA [39].
To test for structural and functional contribution of mitochondrial dysfunction to neurodegeneration in multiple sclerosis (MS).
In order to shed light on this issue, and to establish a model for studying the functional contribution of progranulin to vertebrate development, we undertook the characterization of the biosynthetic origins of progranulins in the zebrafish.
Using those GWAS findings to inform the generation of patient-specific models of AD carrying each of those functional variants will be a powerful tool for testing the functional contribution of those genes and pathways and different cell types to the disease process.
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