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The conserved noncoding elements identified in TFCONES represent a catalog of highly prioritized putative cis-regulatory elements of TF-encoding genes and are candidates for functional assay.
Uptake and/or efflux of isotope-labelled drugs or rhodamine123 are frequently used for functional assay of Pgp-170 in tumor cells.
Conditioned media (CM) were prepared from RA FLS culture with tumor necrosis factor alpha (TNFα) for 24 hours and used for functional assay.
For functional assay, OR2L13 was then transiently expressed in heterologous HEK 293 cells in combination with CRE-SEAP reporter plasmid to monitor SEAP quantity in response of odor stimulation.
The pYEX-CHT expression vector [ 39] was used for functional assay in combination with the double deficient ole1 elo1 S. cerevisiae strain (MATa elo1:: HIS3 ole1:: LEU2 adefectiveleu2 ura3), defectine in both desaturase and elongase gene functions [ 40].
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GeneMANIA (Andersen et al., 2008) generates hypotheses about gene function, analyzing gene lists and prioritizing genes for functional assays.
New methods for functional assays of chemical receptors are highly essential for the research of chemical signal transduction mechanisms and for the development of chemical biosensors.
The second is that the assembly process is highly efficient in vivo, and most factors play important, but non-essential roles, which makes it impossible to isolate factor-specific intermediates for functional assays.
Cells were used for functional assays at day 7.
Clinical data was obtained for all patients and blood collected for functional assays and DNA extraction.
P-tub-Gal4 and 24B-Gal4 drivers were used as controls for functional assays, as appropriate.
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