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These NILs were backcrossed three times and self-pollinated for four to six generations.
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The five family pedigrees used for linkage analysis varied in depth from four to six generations and ranged in size from 72 to 97 animals.
Horticultural protocol requires that a new chili self-pollinate for five to eight generations before it can be considered stable and, therefore, a distinct variety.
I was born and raised in India, where it's common for two to three generations to live together under one roof.
Transgenic founders were crossed for one to four generations before initiating a detailed analysis of expression patterns.
Ghrelin KO mice were kindly provided by Tamas Horvath with permission of Mark Sleeman on a mixed background of C57BL/6 and SJL/J and crossed for one to two generations onto C57BL/6J upon arrival and ovarian transfer rederviation at the Caltech animal facility.
Ntn1 +/− animals used in this study were backcrossed to C57BL/6 animals for three to five generations.
Analyzed knockouts had been backcrossed to this background for three to eight generations.
These strains have been successfully propagated for two to four generations by mass mating.
The AnTc-grown population was then diluted 10-fold and grown for three to four generations without AnTc.
Then the Arf+/− Rosa26-FoxM1b Tg mice were backcrossed for six to nine generations into the C57BL/6 background.
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