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Antisera were used for following experiments after the third immunization.
Moreover, an ozone flow rate of 12 mg/min was chosen as optimum condition for following experiments.
After several purification steps including removal of secondary metabolites using DEAE-Sephacel (Sigma, Steinheim, Germany), the eluted enzyme fraction contained crude phenol oxidase which was used for following experiments.
After 8-day culture, neurosphere were formed and the cells were enzymatically dissociated for following experiments.
The remaining 10 rats from each group were used for following experiments at the end of exposure.
Only animals which have stable blood glucose level more than 15 mmol/l and no loss of weight more than 20% within the first days after onset of diabetes were used for following experiments.
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The resulting cell suspension was stored at 4°C, and used for the following experiments within 2 h.
Surviving mice were monitored for at least 7 days following experiments for signs of intoxication.
We therefore used this ratio for all the following experiments in-vitro.
For all the following experiments, we used this local injection protocol.
For all the following experiments, excitation and absorption wavelengths 488 and 405 nm were used.
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