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Gonadal and renal fat pads were immediately removed and put into tubes containing 37°C phosphate-buffered saline for following analyses.
Three measurements were performed in each tumor during anaesthesia and the mean value was used for following analyses.
Contrast images of transverse section of formaldehyde fixed soleus muscle were taken microscopically for following analyses of cross sectional area of individual muscle fibers.
RNA quality was examined by gel electrophoresis and only paired RNA with high quality was used for following analyses, including RNA-seq.
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For the following analyses, the estimated parameters for the better fitting two-dimensional PCM are used.
A total of 567,485,727 transcript reads accounting for previously described genes were used for the following analyses.
Therefore, we merged the data from these two replicates for all samples and used them for the following analyses.
For similar reasons, we did not adjust for age, methylation method, or sample type for the following analyses.
FastQ files from individual lanes were merged for each sample and served as input for the following analyses.
The high counts/kinds ratio for the high-copy sequ-seqs (counts ≥3) used for the following analyses ensured higher reliability of the reported results.
Thus, I used all the 46 ASEs with the expression level data (data for 2 ASEs were missing due to technical issues) for the following analyses.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com