Allowing an increased range of flux variation, without employing either overexpression or non-native promoters, the tetraploid system is ideal for the determination of flux control coefficients.
Such mutations are linked to fatigue, weakness, ptosis, and exercise intolerance, confirming that ANT1 is a point of flux control for proper metabolism in muscle.
(right) Box plots of flux control coefficient (FCC) for lactate production for Oxygen consumption (OxPhos) and ATP consumption (ATP).
(B ) (left) Box plots of flux control coefficient (FCC) for lactate production for each enzymatic step in glycolysis (FCC = dlnJlac/dln Ei) where Jlac is the rate of pyruvate conversion to lactate, and Ei is the ith enzyme in glycolysis for each step of glycolysis.
Two states of glycolysis were observed with different extents of flux control, tendencies for aerobic glycolysis, and concentration patterns along glycolysis.
For the double knockout, this shift causes a reversal of roles in terms of flux control where the supply block has more control of flux than the demand block.
The resulting transgenic lines then provide material suitable for precise flux control analysis.
These genes are termed HFC for high flux control.
The proposed torque/flux SMC has two integral switching functions for torque and flux control.
