Sentence examples for for five mins from inspiring English sources

Came home and cooked dinner – sweet potato microwaved for five mins, with defrosted homemade chilli and spinach – then more uni work before bed.

Cultures were then incubated with 1 ml trypsin-EDTA (Sigma) for five min at 37°C to produce a cell suspension, the trypsin was then neutralized using DMEM containing 10% FBS and removed following centrifugation for five mins at 1000 RPM (168 RCF).

The PCR program started with an initial step for five min at 95 °C followed by five cycles of 20 s at 95 °C, 20 s at 52 °C, and 60 s at 72 °C.

The cells were fixed in 4% paraformaldyhyde for 15 min followed by three washes with PBS for five min each.

Samples were incubated at room temperature for 10 min; 42°C for 55 min; 95°C for five min using the Reverse Transcription System (Promega).

Saliva was collected immediately following injection for five min and chilled on ice as previously described [14].

The samples were washed in PBS/0.1% Triton X-100 three times for five min before incubation overnight at 4°C with the primary antibody in PBS/0.1% Triton X-100/1 X-100/1

The obtained cell lysate was sonicated two times for five min (50% duty cycle, 1 s on, 1 s off) on ice.

The supernatants were retained, spun for five min and the protein concentration of the samples was measured using the BCA method (Pierce, Cheshire, UK).

Briefly, 5×106 CD4+ T cells were pelleted by centrifugation at 300 g for five min. The supernatant was carefully removed and discarded.

Blood samples were collected using intravenous canula from individuals and immediately centrifuged for five min at 14,000 rpm to separate serum from other cellular materials.