For second passage the dissociated cells were re-plated as above.
The cells were incubated at 37°C for 4 days, and cell culture supernatants were used for second passage on PKE cells.
The homogeneity of epithelial cell population was determined by immunostaining of first passage cultures for surfactant expression (Dako, Captine, CA) [ 20].
The results of our analyses demonstrate that the K/Na ratio was different for each cell passage (4.72 for the first passage, 4.55 for the second passage, and 7.82 for the third passage), suggesting that the highest cell viability corresponds to the third passage.
Scaling laws for the probability density function for a dispersive process, the distribution for the first passage times, the mean first passage time, and the finite-size Lyapunov exponent are presented for fixed points of both deterministic and stochastic renormalization group operators.
The supernatant of brain homogenate was used to inoculate C6/36 cells, and CPE was visible at ≈96 hpi for the first passage and at 72 hpi for second and third passages.
This work aims at an accurate reference solution, providing a basis for the comparison with Monte Carlo based procedures for the first passage problem of non-linear systems.
