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The fixed lines serve as a permanent mapping population for fine mapping and extraction of favourable gene combinations.
Nevertheless, the listed SNPs between N22 and IR64 can be utilised for fine mapping and functional validation of the QTLs.
These NILs are useful material for fine mapping and QTL cloning but also for breeding and for detailed physiological studies.
Understanding the extent of AH may guide the development of new methods for fine mapping and association mapping of complex traits.
A total of 41 plants with extremely low BRRs (BRR < =72.6) were selected for fine mapping and the use of heterozygous plants or confounding plants was avoided.
Moving ahead, cooperation between the groups collecting biospecimens and generating genome-wide data from large numbers of patients with PBC will be essential, not only to increase power for fine mapping and future studies of rare variants and epistasis; but to streamline efforts to perform functional validation of novel discoveries.
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Based on the F12 genotyping results, microsatellite markers used for fine mapping Tir2 and Tir3 loci on MMU1 and MMU5 were mapped and best ordered on each chromosome, using the MapMaker/QTL program [ 25].
The numerous microsatellites will facilitate integration of the linkage and physical maps and serve as valuable resource for fine mapping QTL and positional cloning of genes affecting aquaculture production traits.
For fine mapping, S04056A and S04058 were selected as flanking markers.
Furthermore, these maps can serve as a potential resource for fine mapping QTL and identification of causative mutations underlying QTL for economically important traits.
B. Genomic DNA isolation for individual leaves for fine mapping (see Methods and Results section: "Fine mapping," step 2). 1. Collect a small piece of leaf in a 1.5-mL tube.
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