Sentence examples for for final refinement from inspiring English sources
A better dataset at 2.3 Å resolution was later collected on the native P21 crystal and used for final refinement.
When more particles were included for final refinement, as expected, we could only obtain a high-resolution structure for the 60S subunit.
A final dataset of 115,039 most homogeneous particles were subjected for final refinement to generate a 4.4 Å resolution map through the process of auto-refinement with C2 symmetry imposed (gold-standard FSC 0.143 criteria) (Chen et al., 2013), particle polishing, and post-processing with auto-mask and the manual-bfactor option (B-factor of −60 Å2) in RELION.
After rebuilding using Coot [55] and refinement including twin laws using Phenix [57], the isolated Uba2ufd structure was then used as a guide for final refinement of the Uba2ufd-Ubc9 structure.
As previously stated, the questionnaire to clinicians within the team sought to offer an opportunity for final refinement.
Two types of restraints were combined to generate 2000 initial structures of the Dvl PDZ/Dvl-C peptide complex; 200 of these structures were selected by using NOE-derived restraints, and 100 structures were then obtained for final refinement.
Round 3 was conducted electronically, we disseminated the new draft ethical framework by mail and email to the panelists for final refinements.
The same relative weight between the EM-density and geometric terms that resulted in good fits to the density without overfitting for the DAPT-bound structure was used for the final refinement of all four structures.
It is important to note that the B factor for the protein ligand complex is arbitrarily assigned and fixed to 150 for the final refinement because the relatively resolution of the TibC/ADP-heptose complex (3.9 Å), which is a general practice in protein crystallography.
Consequently, for this final refinement we adjusted one parameter at a time up and down by about 1%.
The SBPkb includes information from the Registry Features table, therefore, for our final refinement, we further restricted our query to return promoters that have sequence annotations of known transcription factor binding sites, i.e., operator sites.
