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However, few aspects warrant special care before a DNA profile is examined for final interpretation.
For final interpretation, each bidirectional sequence was automatically aligned with a panel of more than 100 reference sequences with the GeneLibrarian™ module of the GeneObjects™ software (Siemens Medical Solutions Diagnostics), allowing genotype and subtype assignment based on percent sequence identity.
Although we listed all quotations under various themes, we report those which best represented the views of the respondents for final interpretation.
To ensure the quality of results, data were triangulated to allow comparison across sources and different categories of respondents and subsequently discussed for final interpretation and presentation.
Calling of the results: For each isolate analyzed the genotype was called if no intermediate genotypic markers were present or flagged for final interpretation by an expert if the MFICORR of one or more genotypic markers was in the grey area (intermediate).
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TM was responsible for the examination of the blood samples and for the final interpretation of the obtained data.
Comparison of the Panbio unit values and of final interpretations (positive or negative using manufacturer's criteria) for all ELISAs (Table 1) demonstrated no significant differences, with the exception of the JEV/Dengue Combo IgM ELISA, which showed significantly different results for plasma and serum for the Panbio unit comparison (P = 0.02) but not for the final interpretation (P = 0.5).
Several limitations should be considered for the final interpretation of results.
If there were any uncertainties, a consultant cardiologist was asked for a final interpretation.
The findings from both methods were analysed separately and later consolidated for the final interpretation [ 9].
The results of the tests were read by three independent readers, and the majority result was used for the final interpretation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com