These results show that PA1– 4 are potentially useful for labeling PA implants for fate-mapping.
7 Cre-lox is an extremely powerful tool to label cells permanently and heritably, as required for fate-mapping studies.
Thus the Tbr2 Cre mouse line is better suited for fate-mapping of IPCs as shown by us using immunohistochemistry and reporter mouse lines.
Its potential applications in vivo include studies during development and in the adult where gene expression from defined promoter elements have to be detected or for the fate mapping of tissues or individual cells, for example in clonal studies [ 19, 20].
Our split-Cre system is ideally suited to be combined with standard LoxP-reporter alleles for lineage fate mapping or selective cell ablation in mice with recombination-induced diphtheria toxin expression [17], [32], [42], [43].
The Cre/LoxP system [1] has been extensively used for cell fate mapping [2] [5] and numerous studies exist in which Cre/loxP was used to analyse the cell-type specific function of a gene by conditional gene ablation or induction [6], [7].
Therefore, checking for the reporter EYFP or Cre ectopic expression in the cerebellum shortly (e.g., 24 h) after t.i. was critical for the fate mapping studies.
Tools for genetic fate mapping will be essential to evaluate the progeny of these cells and the long-term homoeostasis of the stem cell compartment(s).
In experiments using inducible Cre lines to activate a permanent genetic label for cellular fate mapping (Kretzschmar and Watt, 2012), Cre toxicity might affect the longevity, proliferation and differentiation of cells, which in turn could influence the results.
For the embryonic induction of AspM-CreERT2/Rosa26YFP double transgenic mice, Tam was intraperitoneally injected into pregnant mice at embryonic days of E10.5//E11.5 and E11.5/E12.5 for short term fate mapping; E12.7 and E13.2 for long term fate mapping.
