Two specimens were tested for acid-fast culture, 1 of which grew M. doricum after 3 weeks of incubation.
"Macaroni is a symbol for fast-food culture," she said, comparing its perils to those of fast fashion.
Three different clones of Lister 427 expressing the tet repressor were compared; these had a slight excess of histone genes, perhaps reflecting long years of selection for fast growth in culture (Fig. 2).
Culture of suspect lesions yielded acid-fast bacilli in ∼11% of cases, with M. bovis accounting for 58 of 171 acid-fast cultures, while 53 isolates were non-tuberculous mycobacteria.
32, 46, 100 In our view, the ideal would be for local diagnostic laboratories to have live neuronal cultures for fast detection of a range of potential NGSAbs and/or live CBAs for identifying the targets; but these live cell assays are time-consuming and costly.
Since March 2009, the Centre Pasteur of Cameroon has performed laboratory confirmation for suspected BU cases: microscopic examination for acid-fast bacilli, culture, and M. ulcerans DNA detection by PCR (6 ).
Samples from 9 of these 12 patients were also sent for acid-fast bacilli cultures, all of which were negative.
Though ocular fluid can be sampled for acid-fast smear, culture, or M. tuberculosis polymerase chain reaction (MTB-PCR), this is often not performed due to low test sensitivity and morbidity related to an invasive procedure.
Of these, 1 recovered spontaneously; 2 remained positive for acid-fast bacilli, culture, or both throughout the study period; and 1 died.
