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The present study was initiated to experimentally optimize the conditions for extraction using Central Composite Design.
Zingiber zerumbet powder (2.0 g) was stirred in aqueous ethanol in preparation for extraction using the microwave extraction system.
The fillet sample were minced, and 10 g in duplicates was used for extraction using chloroform and methanol according to the modified protocol of Bligh and Dyer [22].
40 50 mg of tissue were used for extraction using the mirVana™ miRNA Isolation kit (Applied Biosystems, Carlsbad, CA, USA).
In all, 40 50 mg of tissue was used for extraction using the mirVana miRNA Isolation kit (Invitrogen, Carlsbad, CA, USA).
Five grams of dried powder of this mixture were used for extraction using 50 ml of boiling distilled water for five minutes.
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MagNa pure® extraction versus the standard procedure for extraction used in the SeptiFast® test reduces the total test time from 6 to 3 or 3.5 hours.
The contents of two plates (192 clones) were combined for extractions using QIAprep Spin Miniprep kits (Qiagen, Venlo, The Netherlands).
Fin tissue samples from individuals selected for RAD genotyping were used for DNA extraction using QIAGEN DNeasy 96 kits.
Approximately 10-40 mg of bone powder was used for DNA extraction using a standard protocol for ancient bone samples [ 22].
The method for cAMP extraction using TCA was modified from a protocol for ATP extraction (Gustafsson, 1979).
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