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The optical setup (Fig. 1(a) ) for extended focus OCT imaging is the same as the one previously published [ 18].
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The festival, which is centered around the Fritz Bornemann-designed Haus de Berliner Festspiele, although events take place across the city, has a strong emphasis on dance and theater, but is notable for its extended focus on key artists rather than a broadly eclectic program.
The evolution of the transverse gain for the extended focus is also studied.
The use of extended focused assessment sonography for trauma (eFAST) has become more prevalent in trauma rooms, raising questions about the real "added value" of chest X-rays (CXRs) and pelvic X-rays (PXR), particularly in haemodynamically stable trauma patients.
For each field, extended focus images (fusion of all z stack images) were created and image analysis was performed using ImageJ software (http://rsb.info.nih.gov/ij).
Single Bessel beams furnish an easy way for multiple trapping, although their extended focus makes a full three-dimensional trap unachievable.
Bright field, phase contrast and wide field fluorescence images were taken in a Zeiss Axioplan 2 microscope equipped with a motorized stage and AxioVision™ software package installed (version 4.6.3 including modules for Z-stack recording and extended focus).
These image stacks were used for cell counting or assembled into extended focus photographs; brightness, color, and contrast balanced and assembled into panels with CorelDraw 11 (Corel, Ottawa, Ontario).
The number of spots within the segmented nuclear mask was determined in Volocity (PerkinElmer) using the "find spots" function: an appropriate offset for spot intensity was chosen in "extended focus" view and the minimum distance between spots was set to 0 µm.
A person is likely to write a lot more during that time away from the daily routine, and that extended focus is especially good for bigger projects.
Carnegie Hall outdoes them all, however, with its extended focus on the music of Louis Andriessen.
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