We defined differential expression based on the inter-subtype variability of expression, whereas for calculating differential co-expression we used correlation within a specific subtype.
This can cause that segregating wild-type seeds can be present in the seed bulks used for expression analysis, whereas the oil composition was measured on single seeds.
In the wing margin, cut is required in a cell-autonomous manner for wingless expression, whereas wingless is required for cut expression in neighboring cells (Micchelli et al., 1997).
All in vitro studies used the pTNT vector (Promega), whereas for expression in HeLaM cells, OPG TASK-1 derivatives were clOPG TASK-1pCderivativesvitrogen).
This cellular population was thus composed of a mixture of cells positive and negative for Hoxd9 expression, whereas all cells were negative for Hoxd13.
Cytosolic localisation of staining was considered a measure for STAT expression whereas nuclear staining was considered indicative for STAT activity.
Briefly, false-discovery rate corrected binomial p-value lower than 0.05, together with 2 1 bias, were considered evidence for monoallelic expression, whereas a positive equivalence test was evidence for biallelic expression.
RNU44/RNU48 was used as an endogenous control for miRNA expression, whereas β-actin was used as an endogenous control for mRNA expression.
For microarray analysis, we used samples of 18 cases that were positive for IFNAR2 expression, whereas no samples were available from 2 cases with insufficient quality of RNA.
On the other hand, Basal Cell Carcinomas were negative for C/EBPδ expression, whereas p63 expression was very high.
Importantly, SCCA positivity also correlated with stage of the disease, as 8.6% of Stage III tissue samples tested positive for SCCA expression, whereas only 2.4% and 3.1% of Stage I and II samples, respectively, showed SCCA expression (p = 0.0005) (Table 4).
