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Because of involvement of imprinting in the region, a specific breeding scheme diagramed in supplemental Figure S4 was followed to produce mice for experiments described below.
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The cells were purged in DCC medium for 4 days prior to each experiment described below, then treated with 25 nM AD combined, or not, with the appropriate treatment.
Each experiment described below was conducted in five replicates.
Adult C57/BL6 mice were used for all experiments described below, except for the extraction of MSCs from bone marrow, for which 4-week-old C57/BL6 mice were used.
Exponentially growing cells were used for all experiments described below.
One clone was used for all experiments described below and named IM-PTEC hereafter.
For the experiments described below, each MCS tested directly corresponds to the UCSC Genome Browser coordinates provided in the second column of Table 1.
At 3 weeks after birth, male rats were selected for the experiments described below and were housed three per cage in a temperature- and humidity-controlled colony room, which was maintained on a 12-h light/dark cycle (07:00 to 19 00 h light on) and with food and water provided ad libitum.
for the experiments described below.
The constructed strains listed in Table 2 were used for the experiments described below.
For the experiments described below, the aqueous crude extract was re-suspended in distilled water.
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