For normalization of expression data, the average brightness of the Cy3 and Cy5 channels respectively was used that was calculated from spots within a range from 30%to85%5% of the signal intensity distribution of all spots.
For PCH analysis, the system was calibrated by measuring the average brightness of EGFP free in solution (26, 27).
If the fluorescent proteins are clustered, then the average brightness of a particle will increase, that is, for dimers the brightness would be double, for trimers it would be triple, etc.
The example shown is a search for all monomeric, constitutively fluorescent (non-photochromic) proteins that have an emission maximum between 500 and 560 and a molecular brightness that is at least 1.5-fold higher than the average brightness of all similarly colored (excitation maximum within 30 nm) proteins in the database published since 2010.
It will give the average brightness of three channel of RGB color space.
Another form of animal camouflage uses bioluminescence to increase the average brightness of an animal to match the brightness of the background.
The brightness of GFP complexes scales well with GFP subunits, as demonstrated by the fit of the average brightness of the monomeric, dimer, and trimeric controls (Figure 5).
The average brightness of an empty portion of each picture was considered as the background for that picture (Bkg).
Also, the SD of individual pixel brightness was not reduced by subtracting the average brightness of the image.
> The average brightness of the SLN, expressed in SBR, was 8.3 ± 3.8 and 10.3 ± 5.7 for the groups with and without patent blue, respectively (Fig. 2; Table 2).
