For example, alternatively spliced transcripts of the dystrophin gene can be detected, but the corresponding circRNA is absent (Surono et al., 1999).
For example, alternatively spliced isoforms, or alleles of the same gene, could assemble into separate contigs.
grubii H99, for example, no alternatively spliced form was found for the putative Dicer-encoding genes.
For example, an alternatively spliced transcript of RPS4 lacks splicing of an intron, which results in the loss of a terminal protein domain.
For example, an alternatively spliced αII-spectrin isoform has been shown to play a role in transducing stress-induced signaling by forming a physical link between JNK stress-induced signaling pathway, and GJ function, to influence intercellular communication under stressful conditions [ 73].
For example, all identified alternatively spliced exons contain only between two and seven residues.
For example, consider an alternatively spliced gene for which ESTs occur in just two of the cDNA libraries in dbEST.
For example, TRAB1 can alternatively recognize the two motifs [ 25] and Rap2.4b can take over DREBP and ERF-function if overexpressed [ 27].
The variant-specific primers were carefully designed, for example, flanking the alternatively spliced sequence if available, to further eliminate the possible genomic DNA contamination.
For example, if an alternatively spliced isoform were generated p = 10% of the time, n = 10 transcript sequences would give a 65% probability of observing this event, and 22 transcript sequences would be required to have >90% probability of observing the event.
Importantly, any RNA species could potentially be targeted using this shRNA library (for example, noncoding RNA transcripts, alternatively spliced transcripts, or viral RNAs) in virtually any mammalian cell.
