For every quantitative reverse-transcription (qRT PCR, a dilution series consisting of 4 serial dilutions was used during optimization of the procedure.
For every sample, a reverse transcription reaction lacking Superscript III was included as a negative control.
For every forward and reverse probe pair we calculated the log ratio of the forward/reverse observed intensities.
Parallel tubes were run for every sample without reverse transcription (no-RT controls) to ensure absence of genomic contamination.
For personal care domain's item response, one point was given to "never," and two points were given for "sometime," three points for "mostly," and four points for "every time" but reverse for items 25, 26, 27, 28, and 32.
For occupational activities domain's item response, one point was given to "never," and two points were given for "sometime," three points for "mostly," and four points for "every time" but reverse for items 34 and 35.
Briefly, individual total RNA (1 µg) samples from six rats for each time-point for every group were reverse-transcribed into cDNA using Advantage RT for PCR kit (Clontech, Mountain View, CA, USA).
A converted reference, where every C was replaced by T for forward strand fragments and every G replaced by A for reverse strand fragments was prepared.
For ANG and ACTB, oligo dT was used for reverse transcription.
Tamso thought I should go for a reverse exacta.
