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A simple accurate correlation for evaluating the z-factor that can be linearized has been developed.
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This study proposed two new accurate simple explicit numerical methods for calculating the z-Factor and viscosity of natural gases.
An important parameter for evaluating the reliability and robustness of the high-throughput assay is the Z' factor.
These factors are crucial for evaluating the shear stresses.
Since statistical measures to evaluate a high throughput screen like the Z-factor [24] do not apply to our multidimensional readout, we defined the false positive rate (FPR).
The Z-factor [26] for the assay was 0.55, appropriate for use in screening (Fig. S2).
The z-factor for the screening assay was found to be greater than 0.8 for both assays.
To determine the Z-factor for the assay using F. tularensis MEP synthase, we adjusted the assay volume to accommodate a 96-well plate, fixed the substrate concentration at the KM (104 µM), used a saturating concentration of NADPH (150 µM), and evaluated three separate lots of purified MEP synthase in a series of assays performed over three consecutive days.
Z factors between 0.5 1 predict high-quality screens (Zhang et al, 1999) and the Z-factor for our screen, using MNNG sensitivity, was 0.514.
The Z-factor for this experiment was calculated to be 0.76.
The Z-factor values for each assay are shown in Additional file 4: Table S3.
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