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Actin served as a control for equal loading of proteins.
β-actin served as a reference for equal loading.
The lower panel show methylene blue stained rRNA for equal loading and RNA quality.
It shows that the energetic effect (employing different temperature increments for equal loading of uniaxial stress) can be used to evaluate the cumulative damage caused by any prior loading.
Rabbit polyclonal anti-actin (Sigma) was used as control for equal loading of total cell extracts.
As negative controls and controls for equal loading the endogenous genes Gapdh was used.
α-tubulin was used as a reference protein for equal loading on the gels.
Proteins were quantified by BCA protein assay kit (Pierce) for equal loading.
The same blot was probed with α-tubulin fragment for equal loading of RNA samples.
Bands were normalized to β-actin level to provide a control for equal loading.
α-tubulin antibody was used to control for equal loading and transfer of the samples, as mentioned previously.
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