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We optimized the 3T3-L1 differentiation conditions to obtain a relatively low level of differentiation in the differentiated controls (17%), which allowed for enhanced discrimination of the effects of the test chemicals on adipocyte differentiation (up to 38%and76%6% for BDE-47 and TROG, respectively).
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Such superselectivity could, for instance, enable enhanced discrimination between cells expressing different levels of a target receptor.
In this context, serotyping is, however, of limited discriminatory value, and techniques like PFGE provide enhanced discrimination for outbreak investigations and surveillance purposes.
Thus, future investigations will aim at evolving new DNA polymerase mutants with even more enhanced discrimination for application in methylation specific PCR approaches.[ 29] Supporting information for this article is available on the WWW under http://dx.doi.org/10.1002/anie.org/10.1002/anie
Previous studies from our laboratory and elsewhere have demonstrated enhanced discrimination and accuracy for PFGE in assigning genetic relatedness of some Salmonella and E. coli O157 H7 strains by concatenating up to six different restriction enzyme patterns into single cluster analyses [ 6, 10, 31, 32].
Our results indicate that the evolutionary pressures that gave rise to the avian retina's various adaptations for enhanced color discrimination also acted to fine-tune its spatial sampling of color and luminance.
Our data supported the improved discrimination of MLVA within the uniform phage type DT104 (18 ) and furthermore showed an enhanced discrimination when compared with PFGE for almost all other phage types investigated (Table 1).
Boltzmann enhanced discrimination of receiver operating characteristic.
The final obtained synthesized features were tested with multiple evaluations, demonstrating their enhanced discrimination capabilities over the original FSs.
Previous observations have also suggested that enhanced discrimination of target mutant alleles carrying single nucleotide variations from wild-type allele RNAs is required for ASP-RNAi.
Thus, a streamlined PFGE scheme with enhanced discrimination was desirable.
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