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Transfected unactivated cells accounted for endogenous activity.
Sections 4 μm thick were dewaxed in xylene, rehydrated in alcohol, and blocked for endogenous activity (3% H2O2 and normal rabbit serum).
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Parallel assays with cells transfected with pcDNA3 plasmid (Invitrogen) were conducted as negative controls in every experiment, and subtracted from the experimental absorbances to correct for endogenous activities.
To account for this endogenous activity, pre-implantation images were subtracted from post-implantation images to yield a selective visualization of hydrogel distribution in the stroke cavity and its evolution over 7 days.
Also, our recording conditions may not have been optimal for detecting endogenous activity.
Here, the SC domain size increases in response to ectopic WUS expression, while the OC size, which is a proxy for the endogenous activity of the WUS promoter, is decreased, but not shut down completely (see Figure 6, black dashed curve).
This corroborates that our B4GAT1 assay can be a valuable diagnostic tool for measuring endogenous activity in patient cells and tissues.
For example, the suprachiasmatic nuclei (SCN) of mammals house the neuronal circadian clocks required for the maintenance endogenous activity rhythms (e.g., [3], [4]).
Finally, Huh7 cells stably transfected with a psiCHECK-miR122 Renilla luciferase reporter for endogenous miR122 activity were used to determine the anti-miR activity of PNA in situ synthesized on pore-widened PSi prior to ultrasonic particle formation.
Cooney et al. (2010) suggest that this is a key requirement for PLCζ to attain endogenous activity [ 64, 74, 162].
Control samples were incubated without BglII to monitor for endogenous nuclease activity.
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