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In addition, while BOX-PCR gels can be visualised directly for assessing small numbers of isolates, our methodology for electronic analysis of larger isolate numbers requires purchase of software modules.
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The authors thank Miss Manohari Vellaiswamy for her valuable help with western-blot assays and Bernard Campagna for electronic microscopy analysis.
Tissues intended for electronic microscopy analysis were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer and processed as described previously [13].
We thank S. Meresse for providing us the Salmonella strain SL1344 and A. Darfeuille Michaud for providing us flagellin purified from SL1344 strain and P Gounon for electronic microscopy analysis.
This allowed the observation of EpCAM+/cytokeratin + cells with a DAPI-stained nucleus and no CD45 staining, magnetically maintained in a single plane for electronic image analysis and digitalization.
A Shimadzu UV-1601 spectrophotometer from LaboMed, Inc. was used for electronic absorption spectral analysis.
The "visualization for electronic and structural analysis" software [27] was used to visualize the various crystal structures.
Mice from each group were humanely killed and tumors were harvested 7 days after treatment for transmission electronic microscope analysis under a JEOL 100CX transmission electron microscope (JEOL, Akishima, Japan).
At the ground-floor level, involving SOPs and other regulatory paperwork, validation can be accomplished using a software tool (SciPort) [ 34] for electronic data entry and analysis.
3, 4 Data collection usually depends on paper-based systems, which are often laborious, sent late because of transfer costs involved, may be incomplete, and require transcription for electronic data entry and analysis once at district or national level.
The well-structured spin polarization spectrum validates Spin-HAXPES in the valence energy range as powerful method for bulk electronic structure analysis.
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