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These cis elements are speculated to be responsible for early responses to pathogen attack [ 56], but they also function in the regulation of genes by developmental stimuli [ 57].
Both Obelix and Asterix were identified from a screen for early responses to signals from a grafted organizer (Hensen's node).
A third gene identified by our screen for early responses to a grafted node encodes the neurotrophin receptor, TrkC.
The present study defines three genes as markers for early responses of epiblast cells to signals from Hensen's node, and their regulation.
An initial survey of 5,000 plaques from the original differential screen for early responses to signals from a graft of Hensen's node [35] identified 49 clones showing differential expression between the induced and uninduced libraries.
Our screen for early responses to neural induction signals was designed to identify new markers for the earliest responses to neural inducing signals, upstream of BMP inhibition, which could in turn be used to identify the missing signals.
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Such phase locking may be the mechanism responsible for the occurrence of excess spike synchrony in particular for early response spikes.
Consequently, this accentuates the urgency of identifying a robust method for early response evaluation.
Our results support further investigation of FDG PET as a biomarker for early response to AI therapy.
To the best of our knowledge, this study is the largest study evaluating FLT-PET/CT for early response assessment of chemotherapy-treated CRC patients with liver metastases.
However, molecular changes precede tumour shrinkage, and therefore, there is an increased interest in functional imaging in the search for early response evaluation attributes [2, 3].
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