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Threshold cycle (CT) values for each were separately normalised against CT values for GAPDH, and a relative fold change in expression with respect to a reference sample was calculated by the 2−ΔΔCt method.
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Secondly, data for each site were separately analysed to test for species × provenance effects within each site.
To avoid PCR and sequencing errors, two independent PCR samples for each ecotype were separately sequenced.
The entire procedural time for each procedure was separately measured and recorded for all the patients.
Classification performance for each band is separately computed.
Urine was separately collected for each period.
Weights of these scraped deposits for each component were taken separately for comparison.
These two values, recorded for each dog, were considered separately for the purposes of statistical analysis.
Values for each section were considered separately for the purposes of statistical analysis.
In some cases, the declarations for each state were issued separately or on different days.
The sequences produced for each sample were analyzed separately.
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