Sentence examples for for each tissue file from inspiring English sources

Unique gene map counts were exported from CLC for each tissue file.

At least 87% of the reads from each sample were mapped to the reference transcriptome for each tissue (Additional file 1: Table S1).

We provide a supplementary list of genes with Z-scores in the 3.4 to 3.6 value range for each tissue (Additional file 5).

To accomplish this, the 50 most tissue-specific genes in either larvae or adult (again, based on enrichment compared to the whole organism) for each tissue were identified (Additional file 1 Table S 1, Additional file 1 Table S2, Additional file 1 Table S3 and Additional file 1 Table S4), and their functions (where known) identified from FlyBase or from the literature.

Each set of parental alignments were treated individually in the following manner: For each tissue, accepted_hits.bam files from the three replicates were merged, sorted and indexed using SAMtools [ 40].

Twist1 binding regions associated with expressed genes for each tissue type (Additional files 7, 8 and 9) were analyzed for transcriptional modules containing a transcription factor-binding sequence within +/- 40 base pairs of the E-box consensus site using Genomatix RegionMiner (Additional file 10).

For read and contig statistics for each tissue separately see Additional file 1: Appendix s2.

Assembly statistics for each tissue are in Additional file 1: Table S2.

The sensitivity and specificity for each tissue were calculated (Additional file 4) as follows: for tissue t true negatives (tn) were non- t tissue samples that matched non- t tissues, false negatives (fn) were tissue t samples that matched a non- t tissue, true positives (tp) were tissue t samples that matched t and false positives (fp) were non- t tissue samples that matched t.

To investigate the tissue functions, the tissue-specific unigenes were enriched for the GO categories of each tissue (Additional file 1: Table S2).

Detailed contig expression lists for each tissue are provided in Additional file 9A and B. Approximately 87%% of the contigs (109,281 in Kaspa and 130,297 in Parafield) were expressed in more than one tissue in both genotypes.